منابع مشابه
Automated voltage-clamp technique.
The voltage-clamp electrophysiology method is the gold standard for measuring the function of ion channels. In the past, this technique has had limited applicability in pharmaceutical drug discovery because of its low throughput, steep learning curve, and challenges in standardization of the experiments. Recently, new electrophysiology platforms have been developed, which are based on the use o...
متن کاملVoltage clamp techniques
Much of what we know about the properties of ion channels in cell membranes has come from experiments using voltage clamp. In general, the method allows ion flow across a cell membrane to be measured as electric current, whilst the membrane voltage is held under experimental control with a feedback amplifier. The method was first developed by Cole (1949) and Hodgkin et al. (1952) for use with t...
متن کاملFrom overshoot to voltage clamp
when we were both living in Trinity College, Cambridge. He finished as an undergraduate in 1935 and was a research scholar and, from 1936, a Junior Research Fellow; I came up as an undergraduate in 1935. In the summer of 1939, he went to the marine laboratory at Plymouth to do experiments on the giant nerve fibres of squid. He invited me to join him, which I did at the beginning of August; we l...
متن کاملNovel voltage clamp circuits for ultra low-voltage applications
Voltage clamps for protecting low-voltage DC circuits require a new approach to circuit design. Many clamps commonly used were built for systems of 5-Volts or higher but lower voltage systems require distinctive clamping abilities. The use very low-voltage precision enhancement-mode MOSFETs play a pivotal role in designing voltage clamps in low-voltage
متن کاملVoltage Clamp Experiments in Striated Muscle Fibers
1. Membrane currents during step depolarizations were determined by a method in which three electrodes were inserted near the end of a fibre in the frog's sartorius muscle. The theoretical basis and limitations of the method are discussed.2. Measurements of the membrane capacity (C(M)) and resting resistance (R(M)) derived from the current during a step change in membrane potential are consiste...
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ژورنال
عنوان ژورنال: Scholarpedia
سال: 2007
ISSN: 1941-6016
DOI: 10.4249/scholarpedia.3060